An Assay to Assess Gap Junction Communication in Cell Lines
نویسندگان
چکیده
منابع مشابه
Differential phosphorylation of the gap junction protein connexin43 in junctional communication-competent and -deficient cell lines
Connexin43 is a member of the highly homologous connexin family of gap junction proteins. We have studied how connexin monomers are assembled into functional gap junction plaques by examining the biosynthesis of connexin43 in cell types that differ greatly in their ability to form functional gap junctions. Using a combination of metabolic radiolabeling and immunoprecipitation, we have shown tha...
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Communication plays an important role in the rapid Connexin Structure progress of modern society. We live in an age when Gap junctions exhibit a hierarchy of assembly. The prininformation is transmitted through many different pathcipal structural component, the membrane protein conways and in many different forms to influence our daily nexin, is organized into the basic unit of structure, the d...
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BACKGROUND/AIMS In the current study we examined if the adipocytokine, visfatin, alters connexin-mediated intercellular communication in proximal tubule-derived epithelial cells. METHODS The effects of visfatin (10-200ng/mL) on cell viability and cytotoxicity in HK2-cells were assessed by MTT, crystal violet and lactate dehydrogenase assays. Western blot analysis was used to confirm expressio...
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ژورنال
عنوان ژورنال: Journal of Biomolecular Techniques : JBT
سال: 2019
ISSN: 1524-0215,1943-4731
DOI: 10.7171/jbt.19-3001-001